Effect of aspirin on apoptosis of human cervical cancer cell line in vitro

Objective To investigate the effect of aspirin on apoptosis of human cervical cancer cell line(Hela) and expression of Bcl-2 and bax genes.Methods The Hela cells were cultured and induced by aspirin in different concentrations(1.0,5.0,10.0 mmol/L).The effect of aspirin on apoptosis of Hela cell and the changes of cell cycle were observed by flow cytometry(FCM).The levels of bcl-2 and bax mRNA were detected by RT-PC.The expressions of bcl-2 and bax in Hela cell line were detected by immuneohistochemistry.Results As compared with that in control group,the apoptosis rate of Hela cells treated with different doses of aspirin was significantly increased(P0.05).The Hela cells at G0/G1 stage were decreased,however,which at G2/M stage were increased(P0.01).The expression of bcl-2 and bax mRNA in Hela cells treated with different doses of aspirin was significantly decreased,however,the expression of bax mRNA was significantly increased,as compared with those in control group(P0.05).After treated by aspirin for 48h,the expression of bcl-2 protein was down-regulated,but the expression of bax protein was up-regulated(P0.05).Conclusion The aspirin can induce apoptosis of Hela cells,and its action mechanism is correlated with inhibition of bcl-2 expression and up-regulating expression of bax.