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Determination of creatinine in human sera with micellar electrokinetic capillary electrophoresis by the homemade instrument

Lin Hong

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Abstract

Objective:To develop a new method for determining creatinine in humanserum using capillary electrophoresis(CE)by the homemade instrument.Methods:Creatinine in human serum was determined with micellar electrokinetic CE and detected at235nm.The running buffer for analyses was120mmol/L SDS_20mmol/L borate,pH9.0.Results:The linear conˉcentration of creatinine was25.0～1600μmol/L with detection limit of12.3μmol/L.The CV values for within_run_assay and within_day_assay were all less than5%.The recoveries of creaˉtinine at different levels were96.0%,99.4%and96.5%,respectively.Comparing this method(Y)with Jaffe method(X),it obtained the regression equation.Y=0.971X-16.48,r=0.9977.Urea,uric acid,caffeine,vitamin C,hemoglobin,bilirubin and lipoprotein had no interfernce with this procedure.Conclusion:CE method is simple,rapid,separation efficient and suitable for the analysis of creatinine in serum clinically.

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Objective:To develop a new method for determining creatinine in humanserum using capillary electrophoresis(CE)by the homemade instrument.Methods:Creatinine in human serum was determined with micellar electrokinetic CE and detected at235nm.The running buffer for analyses was120mmol/L SDS_20mmol/L borate,pH9.0.Results:The linear conˉcentration of creatinine was25.0～1600μmol/L with detection limit of12.3μmol/L.The CV values for within_run_assay and within_day_assay were all less than5%.The recoveries of creaˉtinine at different levels were96.0%,99.4%and96.5%,respectively.Comparing this method(Y)with Jaffe method(X),it obtained the regression equation.Y=0.971X-16.48,r=0.9977.Urea,uric acid,caffeine,vitamin C,hemoglobin,bilirubin and lipoprotein had no interfernce with this procedure.Conclusion:CE method is simple,rapid,separation efficient and suitable for the analysis of creatinine in serum clinically.

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Available abstract

Objective:To develop a new method for determining creatinine in humanserum using capillary electrophoresis(CE)by the homemade instrument.Methods:Creatinine in human serum was determined with micellar electrokinetic CE and detected at235nm.The running buffer for analyses was120mmol/L SDS_20mmol/L borate,pH9.0.Results:The linear conˉcentration of creatinine was25.0～1600μmol/L with detection limit of12.3μmol/L.The CV values for within_run_assay and within_day_assay were all less than5%.The recoveries of creaˉtinine at different levels were96.0%,99.4%and96.5%,respectively.Comparing this method(Y)with Jaffe method(X),it obtained the regression equation.Y=0.971X-16.48,r=0.9977.Urea,uric acid,caffeine,vitamin C,hemoglobin,bilirubin and lipoprotein had no interfernce with this procedure.Conclusion:CE method is simple,rapid,separation efficient and suitable for the analysis of creatinine in serum clinically.

Key concepts: Creatinine, Chromatography, Chemistry, Capillary electrophoresis, Uric acid, Detection limit, Electrokinetic phenomena, Bilirubin

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