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Application of Suppression Subtractive Hybridization in Soybean Research

Hou Wen-sheng

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Abstract

Suppression subtractive hybridization(SSH),which has been widely used in medical research,is an effective approach to identify genes that vary in expression levels,SSH was also applied in plant research widely because of its low false positives,high sensibility and high efficiency.In recent years,SSH was used in soybean researches relating to soybean mosaic virus,phytophthora,photoperiodism,abiotic stresses,etc.In this review,the principle,characteristics,improvement of SSH and the application of SSH was introduced and summarized in soybean researches.

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What this paper is about

Suppression subtractive hybridization(SSH),which has been widely used in medical research,is an effective approach to identify genes that vary in expression levels,SSH was also applied in plant research widely because of its low false positives,high sensibility and high efficiency.In recent years,SSH was used in soybean researches relating to soybean mosaic virus,phytophthora,photoperiodism,abiotic stresses,etc.In this review,the principle,characteristics,improvement of SSH and the application of SSH was introduced and summarized in soybean researches.

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Available abstract

Suppression subtractive hybridization(SSH),which has been widely used in medical research,is an effective approach to identify genes that vary in expression levels,SSH was also applied in plant research widely because of its low false positives,high sensibility and high efficiency.In recent years,SSH was used in soybean researches relating to soybean mosaic virus,phytophthora,photoperiodism,abiotic stresses,etc.In this review,the principle,characteristics,improvement of SSH and the application of SSH was introduced and summarized in soybean researches.

Key concepts: Suppression subtractive hybridization, Soybean mosaic virus, Biology, False positive paradox, Phytophthora, Abiotic component, Subtractive color, Biotechnology

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