Applied Study on Detection of PLASMODIUM in Blood Samples by Multiplex Polymerase Chain Reaction

Objective] To study the multiplex PCR for suitable to detect Plasmodium in field. [Methods] The samples were treated with Normal saline (N.S),malaria parasites DNA were amplified by the multiplex PCR using two sets of primers from the P.f moderately repetitive DNA sequence pRBK 1 14 and Pv COIII gene . [Results] A 210 bp product for P.f and 370 bp product for P.v were amplified by multiplex PCR, no special products were amplified for P.c, P.b and human WBC. P.f and P.v samples from different areas show the same amplified results, the method can detect parasitemia level as low as 5.5×10 -7 for P.f and 1. 5×10 -6 for P.v. A total of 146 fever samples were detected,the sensitivity and specificity were 100% and 98 7% for P.v, 97 9% and 98.7% for P.f. [Conclusion] The multiplex PCR can be suitable for The field malaria epidemiological surveillance, evaluation of the control effect ,diagnosis and determination of malaria species.