Preparation of T-2 Toxin Artificial Antigen and Mouse Polyclonal Antiserum

To prepare high sensitive and specific polyclonal antiserum against T-2 toxin,T-2 toxin artificial antigens were synthesized and identified by animal immunization in this study.T-2 toxin was converted to T-2-hemisuccinate(T-2-HS) and coupled with carrier pro tein BSA and OVAusing EDC method,and then the immune antigen T-2-BSA and detection antigen T-2-OVA were obtained,respectively.To prepare the poly-antiserum against T-2,BALB/c mouse was immunized with T-2-BSA at the doses of 30 μg and 50 μg for four times at the intervals of four weeks.Ten days after the last immunization,the titer of antiserum was determined by indirect ELISA.The results showed that antiserum of high titers above 1∶104 were produced in six mice.The antiserum of mouse #2 had the best sensibility.Its IC50 was 324 ng/mL and it had good specificity.In this study,T-2 toxin artificial antigens were successful synthesized and the poly-antiserum with high sensibility and specificity was obtained,providing a foundation for the further study.