HPLC-UV specific chromatogram study and determination of seven organic acids from Datusizi( Cuscutae Japonicae Semen) before and after salt processing

Objective: To develop the HPLC-UV specific chromatograms of Cuscutae Japonicae Semen before and after salt processing,to explore the regularity of component changes in processing of Cuscutae Japonicae Semen,and conduct quantitative analysis and compare neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,coffeic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid. Methods: HPLC-UV was used,and the separation was performed on an Odyssil C18column( 4. 6 mm × 250 mm,5 μm),the mobile phase was acetonitrile( A)-0. 05% phosphoric acid( B) in gradient elution( 0-5 min,5% A→13% A; 5-20 min, 13% A→13% A; 20-25 min,13% A→20% A; 25-45 min,20% A→40% A),at the flow rate of 1. 0 mL·min-1, the column temperature was set at 35 ℃ and the detection wavelength was at 325 nm. The data were analyzed with similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2004A). Results: The linear correlation of major components was good( r≥0. 9970). HPLC-UV specific chromatograms of Cuscuta Japonica Semen from different areas were highly similar,while the differences were obvious in contents of major components,and the contents of neochlorogenic acid,cryptochlorogenic acid,3,4-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid increased exponentially after processing; on the contrary,the contents of chlorogenic acid,coffeic acid,and 3,5-dicaffeoylquinic acid decreased exponentially. The similarity test by fingerprint found the difference between raw products and salt products was relatively greater,while the difference between raw products and pharmacy products was the minimal. Conclusion: The HPLC-UV specific chromatogram and content determination of seven organic acids can be used for the quality control and distinction of Cuscutae Japonicae Semen before and after salt processing.