Study on Interaction between Azathiprine and Human Serum Albumins

The binding characteristics between azathiprine and human serumalbumin was studied by fluorescence spectroscopy.The equilibrium constant,K, and the number of binding sites, n ,have been measured .The effect of azathiprine on the conformation of HSA was also analysited using syuchronous fluorescence spectroscopy. The combination reaction of azathiprine with HSA is a single static quenching process and azathiprine can effect the conformation of human serum albumin.