The Reaction Mechanism between Cefaclox and Human Serum Albumin

To study the reaction mechanism between cefaclox and human serum albumin(HSA) in aqueous solution, fluorescence spectra were used. The interaction dissociation constants of human serum albumin and cefaclox were determined from a double reciprocal Lineweaver-Burk plot. The effect of cefaclox on the conformation of HSA was analyzed using synchronous fluorescence spectrometry. The binding distance and transfer efficiency between cefaclox and HSA were also obtained according to the theory of Forster′ non-radiation energy transfer. The main binding force between cefaclox and HSA is hydrophobic interaction. The antibiotic valence is related to the transfer efficiency and dissociation constant.