Expression of osteopontin in laryngeal carcinoma tissues and effect of LV-shOPN on infiltration and metastasis of laryngeal carcinoma Hep-2 cells

Objective To observe the expression of osteopontin(OPN) in the largngeal squamous cell carcinoma(LSCC) tissues,and the effect on the proliferation and invasiveness of laryngeal cancer Hep-2 cells of lentivirus vector with interference OPN(LV-shOPN).Methods SP immunohistochemical method was used to detect the OPN of 44 LSCC and adjacent tissues.LV-shOPN was transfected into Hep-2 cells.OPN in the Hep-2 cells was detected by Western blot was detected by Transwell assay,the proliferation of Hep-2 cells was examined by MTT.The invasion ability of Hep-2 cells was detected by Transwell assay.Results The positive rate of OPN in LSCC and adjacent tissue was 75.0 %(33/44)and 13.6%(6/44)respectively,the two rates were compared,P0.01.The OPN expression levels were significantly correlated with the presence of lymph node,clinical stage and histopathology grade of LSCC,all P0.01.After LV-shOPN transfection,the expression of OPN was reduced and ability of the proliferation and invasiveness was decreased in Hep-2 cells(all P0.05).Conclusions OPN expression is higher in LSCC than that in adjacent tissue,and maybe participated in the development and metastasis of LSCC.LV-shOPN can inhibit efficiently the proliferation and invasiveness of Hep-2 cells.