2002•Laboratory Animal Science and AdministrationRequires access
Bile Duct Occlusion-induced Liver Fibrosis in Rats
Bao-en Wang
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Abstract
objective To establish liver fibrosis model induced by bile duct occlusion in rats.Method The bile ducts of female Wistar rats were completely occluded by retrograde injection of cyanoacrylate tissue adhesives and ligation.Serum ALT,AST,HA,LN were determined on days 28,56 following the occlusion.Livers were examined for histopathological changes on 56 days.A sham-operation group was used as a control.Results Serum ALT,AST,HA,LN levels of model group were highter than that of control group (P0.001).Profound fibrosisi surrouding hyperplastic bile ductules were seen in model group.Conclusion Bile duct ligation could induce stable and reproducible fibrosis,and may serve as a convenient procedure for screening antifibrotic agents.