Preparation and Quality Evaluation of Brucine Solid Lipid Nanoparticles

Objective: To prepare brucine solid lipid nanoparticles (Bru-SLN) by means of emulsification-solvent evaporation method and to evaluate its quality. Methods: The formulation of Bru-SLN were optimized by the orthogonal design on the basis of single factor exploration. The morphology was examined by the transmission electron microscope. High-performance liquid chromatography was employed to determine the entrapment rate of Bru-SLN. The diameter, zeta potential and the stability of Bru-SLN were investigated by laser light scattering analysis instrument. Results: The obtained solid lipid nanoparticles were round, smooth particles with average size of 116 nm, zeta potential - 29.98 mV and entrapment rate 50.7%. There were no notable changes of the particle size and entrapment rate at 4 ℃ within one month. Conclusion: Bru-SLN shows a narrow particle size distribution and good stability, which can be a promising low-toxicity and long-effect preparation.