Prokaryotic Expression and Biological Activity of Recombinant Human IL-17B/His Protein

Objective To prepare recombinant human IL-17B/His protein and investigate its biological activity in vitro.Methods The gene region of human IL-17B was cloned by RT-PCR.After identification by sequencing,the hIL-17B gene encoding function domain was cloned into expression plasmid PQE3.0 and transfected into E.coli M15.By the induction of Isopropyl-β-D-Thiogalacto-Pyranoside(IPTG),recombinant IL-17B/His protein was effectively expressed in E.coli M15.The recombinant protein was identified by Western blot.Results After renaturation and purification by HiTrapTM affinity column,the recombinant proteincan up-regulated macrophages to secret TNF-α,IL-1β and other relative cytokines in vitro.Conclusion hIL-17B/His recombinant protein is of high biological activity,which can be used to make further study of its special characteristic.