Construction and expression of the recombinant prokaryotic expression vector with hIL-24

In this study,a recombinant expression vector of human interleukin-24(hIL-24) gene,named pET-28a(+)-hIL24,was constructed by gene cloning.The recombinant protein hIL-24 was expressed in E.coil and analyzed by SDS-PAGE and Western blotting.The proper inducing conditions of its expression were explored.The results showed that the recombinant protein was about 25 ku and mainly existed in the precipitation after sonication.Western blotting proved that the recombinant protein IL-24 was recognized by mouse anti-human IL-24.SDS-PAGE analysis indicated that the best expression condition was 0.5 mmol·L-1 IPTG for 8 h at 37 ℃.The successful expression of hIL-24 in the prokaryotic expression system is helpful for the further study of its biological function.