The relationship between PCNA and cell apoptosis in human breast cancer

Objective:To investigate the relationship between proliferating cell nuclear antigen(PCNA) and cell apoptosis in human breast cancer.Methods:MTT colorimetric assay was applied to examine the growth inhibition,and apoptosis rates were determined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling(Tunel);The expressive levels of dephosphorylated RB protein and PCNA were detected with immunocytochemistry.Results:MTT assay that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration (IC_50 ) was 0.128mg/L.Tumor cell apoptotic rate(AR) in ADR group(=0.261) was significantly higher than that in control group (=0.0449,P0.01),PCNA positive expression rate in ADR group (=0.3371) was significantly lower than that in control group (=0.5152,P0.01).Conclusion:There was significant negative association between AR and PCAN in ADR group and control group;ADR could include MCF-7/S cells apoptosis and inhibit MCF-7/S cells proliferation.