Effect of Adriamycin on Cell Apoptosis and Proliferation in Human Breast Cancer

To investigate the effects of adriamycin(ADR)on the apoptosis and proliferation of human breast cancer cells(MCF-7/S)cultured in vitro and to discuss the possible mechanism.MTT colorimetric assay and flow cytometry(FCM)were applied to examine the growth inhibition and apoptosis respectively.The expressive levels of dephosphorylated Rb protein and proliferating cell nuclear antigen(PCNA)were detected with immunocytochemistry in MCF-7/S cells.ADR inhibited proliferation of MCF-7/S cells in a dose-dependent manner.Tumor cell apoptotic rate (AR) and the expressive level of dephosphorylated Rb protein in ADR group were significantly higher than those in control group(P0.01).PCNA positive expression rate in ADR group was significantly lower than that in control group(P0.01).There was significant positive correlation between AR and the expressive level of dephosphorylated Rb protein in ADR group,but there was significant negative correlation between AR and PCNA.[Conclusions]ADR induces apoptosis and inhibits the growth proliferation in MCF-7/S cell,which is possibly related to the expression of ascending dephosphorylated Rb protein and descending PCNA.