Cloning of VP1 and VP3 genes of DHV and construction of their baculovirus expression vectors

VP1 and VP3 genes of duck hepatitis virus were amplified by RT-PCR and cloned into the pGEM-T vector. After certified by sequencing and being digested with BamHⅠand XhoⅠ respectively,the genes were linked to the baculovirus transfer vector pFastBac1. In result,the recombinant vectors pFastBac1-VP1 and pFastBac1-VP3 were constructed successfully.Then the recombinant vectors were transformed into DH10Bac E.coli,with the positive recombinant bacmid rBacmid-VP1 and rBacmid-VP3 screened according to the resistant and the blue-white plague screening. The study provided theoretical and practical foundations for the expression of VP1 or VP3 genes in insect cells.