Expression of VP1 and VP2 genes of chicken anemia virus by baculovirus expression systems

Chicken anemia virus strain M9905 was isolated in Mudanjiang region of Heilongjiang province of China in 1999. It's VP1 and VP2 genes encoded by open reading frame (ORF) 1 and 2 were cloned and inserted into the baculovirus transfer vector pFastBacDUAL and then recombined with the baculovirus shuttle vector Bacmid by transforming DH10BAC competent cells. Finally, Sf9 cells were transfected by recombinant Bacmid DNAs and three recombinant viruses that respectively contained VP1,VP2 or VP1+VP2 gene(s) were obtained. PCR amplification confirmed that VP1 and VP2 genes had inserted into the baculovirus genome. Indirect immunofluorescence assay and SDS-PAGE analysis showed that vp1 and vp2 has been expressed in Sf9 cells infected with the recombinant baculoviruses.