Studies on the genetic variation of main characters of Oryza sativa progenies derived from anther culture in vitro

Both callus induction frequency and differentiation ability of calli (green plants regeneration ability) were significantly influenced by the material genotype. However, the green plants regeneration ability of 94P-70 was rather higher than other materials, the second was Shanyougui 99. Other materials did not produce green plant. First generation (H_(1)) of pollen-plant showed wide range of characters variation. Some characters of many strains of the second generation (H_(2)) were relatively stable and better than that of the first generation (H_(1)); Comparatively, the second generation (H_(2)) revealed better agronomic characters than their parents but a few strain shows segregation. Polyacrylamide gel electrophoresis analysis of peroxidase and esterase isoenzyme patterns of the H_(2) of pollen-plants were equal to that of the parents (relatively stable) but one strain of the second generation (95EH-19-5) was significantly different from that of the parents.