ScholarLens Back to search
2009•Unpublished venueRequires access

On the Detectionamino of Amino Acids Neurotransmitter in Rat Brain by RP-HPLC

Song Tai

Open publisher page 1 citations

Abstract

Established a method for the determination of amino acids neurotransmitter in rat brain by RPHPLC. The samples were pre-column derivated with DNFB,Diamonsil C18column(4.6×200mm,5μm),acetonitrile /acetate buffer solu-tion as mobile phase,gradientelution with UV 350nm detection.According to the study, amino acids were separated within 18 mins.The linear re-lation was well in the range from 0.01 to 100ug/ml,The correlation coefficients were:Asp0.9985,Glu0.9976,Gly 0.9943,GABA0.9924 respectively.The precisions were Asp2.4%,Glu3.8%,Gly 3.2%and GABA3.5%for interlay:The average recoveries of amino acid were Asp 95. 18%,Glu 94.70%,Gly 97.60%and GABA95.90%respectively.The method was speediness,accurate,simple, high sensible and well repetition,and supplied a reference for the clinic determination and scientific research.

About this research paper

What this paper is about

Established a method for the determination of amino acids neurotransmitter in rat brain by RPHPLC. The samples were pre-column derivated with DNFB,Diamonsil C18column(4.6×200mm,5μm),acetonitrile /acetate buffer solu-tion as mobile phase,gradientelution with UV 350nm detection.According to the study, amino acids were separated within 18 mins.The linear re-lation was well in the range from 0.01 to 100ug/ml,The correlation coefficients were:Asp0.9985,Glu0.9976,Gly 0.9943,GABA0.9924 respectively.The precisions were Asp2.4%,Glu3.8%,Gly 3.2%and GABA3.5%for interlay:The average recoveries of amino acid were Asp 95. 18%,Glu 94.70%,Gly 97.60%and GABA95.90%respectively.The method was speediness,accurate,simple, high sensible and well repetition,and supplied a reference for the clinic determination and scientific research.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Established a method for the determination of amino acids neurotransmitter in rat brain by RPHPLC. The samples were pre-column derivated with DNFB,Diamonsil C18column(4.6×200mm,5μm),acetonitrile /acetate buffer solu-tion as mobile phase,gradientelution with UV 350nm detection.According to the study, amino acids were separated within 18 mins.The linear re-lation was well in the range from 0.01 to 100ug/ml,The correlation coefficients were:Asp0.9985,Glu0.9976,Gly 0.9943,GABA0.9924 respectively.The precisions were Asp2.4%,Glu3.8%,Gly 3.2%and GABA3.5%for interlay:The average recoveries of amino acid were Asp 95. 18%,Glu 94.70%,Gly 97.60%and GABA95.90%respectively.The method was speediness,accurate,simple, high sensible and well repetition,and supplied a reference for the clinic determination and scientific research.

Key concepts: Neurotransmitter, Amino acid, Chromatography, High-performance liquid chromatography, Acetonitrile, Chemistry, Receptor, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
On the Detectionamino of Amino Acids Neurotransmitter in Rat Brain by RP-HPLC — Research Paper | ScholarLens