2009•He'nan nongye kexueRequires access
Determination of 4 Amino Acids Neurotransmitters in Rat Hippocampi by RP-HPLC
Fan Zhang
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Abstract
In this paper,a method for the determination of 4 amino acids neurotransmitters in rat hippocampi by RP-HPLC was established.The samples were precolumn derivated with DNFB.The chromatogram condition is Diamonsil C18 column(4.6mm×200mm,5μm).Acetonitrile/acetate buffer solution was used as mobile phase and the gradient elution was detected by UV at 350 nm.The results showed that 4 amino acids neurotransmitters were separated within 18 minutes.The linear relation was well in the range from 0.01 to 100μg/mL,and the correlation coefficients of Asp,Glu,Gly and GABA were 0.9985,0.9976,0.9943,and 0.9924,respectively.The coefficient of differentiations were 2.4%,3.8%,3.2% and 3.5%,and the average recoveries were 94.80%,93.93%,92.69%and 96.74%,respectively.This method is speediness,accurate,simple,high sensible and well repetition and provides a reference for the determination of amino acids neurotransmitter and related researches.