2001•Europe PMC (PubMed Central)Requires access
DNA fingerprinting of Castanea mollissima elite variety (clone) seedlings
Weng Yaofu, Chen Yuan, Zhao Yongchun, Zheng Kangle, Shao-Fu Huang
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Abstract
RAPD----based fingerprinting was carried out for 8 varieties and clones of Castanea mollissima which had been approved by the Zhejiang Inspection Committee of Superior Tree Species. Each of the varieties (clones) was identified by fingerprints. Nine RAPD markers were obtained from repeated screening of 684 random oligonucleotide primers and DNA fingerprints were made for all the used varieties (clones). Stable amplification was obtained through modification of experimental conditions and a complete technical system was established for fingerprinting of Castanea mollissima. Preliminary results for verification of commericial seedlings showed that these fingerprints were reliable.