Green Synthesis of Silver Nanoparticles using Cinnamomum Zylinicum and their Synergistic Effect against Multi-Drug Resistance Bacteria

Multidrug - resistant (MDR) bacteria are considered life-threatening and need fast identification and antibiotic sensitivity testing to overcome this problem. In the current article, we highlighting the potential of developing silver Nanoparticles (AgNPs) using Cinnamomum zylinicum bark extracts as a promising approach. UV, SEM, TEM and FT-IR analysis were carried out to characterize the biosynthesized AgNPs. UV-visible spectroscopy showed the presence of characterized peek at (420 nm), TEM showed spherical shaped and monodispersed nanoparticles of size range 10 to 78.9 nm and FT-IR spectrum confirmed the presence of various functional groups in the biomolecules which serve as a capping agent for the nanoparticles. Biosynthesized (AgNPs) have been evaluated as an antibacterial against MDR gram-negative bacteria Acinetobacter baumanni, Klebsiella pneumoniae, and Pseudomonas aeruginosa strains and gram-positive bacteria Staphylococcus aureus. The results showed that obtained silver Nanoparticles are efficient in inhibiting both gram-positive and gram-negative bacteria when compared with antibiotic giving a zone of inhibition of 25 mm against S. aureus, 24 mm against K. pneumonia, and P. aeruginosa and 22 mm against A. baumanni respectively. Furthermore, the effectiveness of AgNPs against these test strains was assessed with multiple broad spectrum antibiotics. The results demonstrated that the incorporation of antibiotics with AgNPs has amazing antibacterial effects. The highest extent was observed with gentamycin against S. aureas, K. pneumonia, P. aeruginosa and A. baumanni, respectively. The minimum inhibitory concentrations (MICs) of AgNPs were also determined using microdilution assay. This study gives encouragement that AgNPs can be used to improve the effectiveness of the current antibiotics against MDR bacteria.