2019•ROMANIAN BIOTECHNOLOGICAL LETTERSOpen access

Characterisation of ESBL producing E. coli strains isolated from chicken meat

Rodica Tanasuica, Florica Bărbuceanu, Isabela Madalina Dragoi Nicorescu, Rodica Claudia Dumitrache, ANGELA MIHAIL, Ștefania‐Felicia Bărbuceanu, Gabriel Predoi

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Abstract

The dissemination of antibiotic-resistant bacteria through the food chain is a matter of concern.Food-producing animals are the primary reservoir of zoonotic pathogens and the rate of detection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in last years.The aim of this study was to analyze the genotypic and phenotypic properties of ESBL-producing E. coli strains from chicken meat and establishing a relationship between isolates.ESBL-producing isolates mentioned in the present study were found to be resistant to multiple drugs, such as extended-spectrum cephalosporins, gentamicin, chloramphenicol, tetracycline, nalidixic acid, ciprofloxacin and sulfamethoxazole.Additionally, our results clearly showed that there are some similarities between genes and bacterial properties in isolates coming from the different populations.

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The dissemination of antibiotic-resistant bacteria through the food chain is a matter of concern.Food-producing animals are the primary reservoir of zoonotic pathogens and the rate of detection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in last years.The aim of this study was to analyze the genotypic and phenotypic properties of ESBL-producing E. coli strains from chicken meat and establishing a relationship between isolates.ESBL-producing isolates mentioned in the present study were found to be resistant to multiple drugs, such as extended-spectrum cephalosporins, gentamicin, chloramphenicol, tetracycline, nalidixic acid, ciprofloxacin and sulfamethoxazole.Additionally, our results clearly showed that there are some similarities between genes and bacterial properties in isolates coming from the different populations.

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Available abstract

The dissemination of antibiotic-resistant bacteria through the food chain is a matter of concern.Food-producing animals are the primary reservoir of zoonotic pathogens and the rate of detection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in last years.The aim of this study was to analyze the genotypic and phenotypic properties of ESBL-producing E. coli strains from chicken meat and establishing a relationship between isolates.ESBL-producing isolates mentioned in the present study were found to be resistant to multiple drugs, such as extended-spectrum cephalosporins, gentamicin, chloramphenicol, tetracycline, nalidixic acid, ciprofloxacin and sulfamethoxazole.Additionally, our results clearly showed that there are some similarities between genes and bacterial properties in isolates coming from the different populations.

Key concepts: Nalidixic acid, Tetracycline, Ciprofloxacin, Biology, Gentamicin, Microbiology, Escherichia coli, Chloramphenicol

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