The effects of Bifidobacterium on the expression of CD86 and HLA-DR in dendritic cells from the children with allergic asthma.

Objective To study the effects of Bifidobacterium on the expression of CD86 and HLA-DR in dendritic cells(DCs) derived from peripheral blood mononuclear cells(PBMCs) of the children with allergic asthma.Method The PBMC-derived DCs were generated in the presence of granulocyte macrophage-colony stimulating factor(GM-CSF) and interleukin-4(IL-4) from 12 patients with allergic asthma and 10 normal children,and then the immature DCs were cocultured with the Bifidobacterium adolescent or Lipopolysaccharide(LPS) for 48 h.The expression of CD86 and HLA-DR of DCs were measured by flow cytometer.Result After the pretreatment with Bifidobacterium adolescent,the expression of CD86 of DCs from the allergic patients was significantly increased(P0.05),but the expression of HLA-DR was not changed(P0.05).The pretreatment with Bifidobacterium adolescent had no effects on the expression of CD86 and HLA-DR on the healthy children.The pretreatment with LPS may increase the expression of CD86 and HLA-DR from both the allergic patients and healthy children significantly(P0.05).Conclusion Both Bifidobacterium adolescent and LPS could stimulate the mature of DCs.The expression of CD86 of DCs from the patients with allergic asthma may be impaired.The Bifidobacterium adolescent may up-regulate the expression of CD86 appropriately.