Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue

Background: This study aimed to evaluate the effects of mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Material and Methods: Human tenon fibroblasts from a patient who underwent socket reconstruction surgery were cultured in vitro. Cell cultures were exposed to 0.2, 0.4, and 1 mg/ml of mitomycin C solution for 5 min, and serum-free culture medium was used as a control. Proliferation was observed with the MTT cell proliferation assay 24 h after treatment. Results: A 5 min exposure to mitomycin C at 0.2, 0.4, and 1 mg/ml caused inhibition of fibroblast proliferation (p Conclusion: Mitomycin C can inhibit tenon fibroblast proliferation of contracted socket tissue. Dose escalation of mitomycin C is not related to the rate of fibroblast proliferation inhibition.