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2003•Journal of the Korean Ophthalmological SocietyRequires access

Inhibitory Effects of Taxol and Mitomycin C on Proliferation of Cultured Rabbit Subconjunctival Fibroblasts

Yong-Woo Baek, Jung-Il Moon, Myung-Douk Ahn

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Abstract

Purpose: Scar tissue formation by proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated inhibitory effects of Mitomycin C (MMC) and Taxol on proliferation of cultured rabbit subconjunctival fibroblasts. Methods: Tenon's fibroblasts of rabbits were cultured and exposed to various concentrations of each drug for 6 days. Cell viability was measured using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumm bromide) assay. Results: Both MMC and Taxol inhibited proliferation of fibroblasts significantly in a dose-dependent manner, with ID50s of 0.27 m/l and 0.08 m/l, respectively. Conclusions: We have confirmed that Taxol is as potent inhibitor of fibroblast proliferation as Mitomycin C. This result may possibly support the use of Taxol in trabeculectomy to improve the success rate of trabeculectomy.

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Purpose: Scar tissue formation by proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated inhibitory effects of Mitomycin C (MMC) and Taxol on proliferation of cultured rabbit subconjunctival fibroblasts. Methods: Tenon's fibroblasts of rabbits were cultured and exposed to various concentrations of each drug for 6 days. Cell viability was measured using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumm bromide) assay. Results: Both MMC and Taxol inhibited proliferation of fibroblasts significantly in a dose-dependent manner, with ID50s of 0.27 m/l and 0.08 m/l, respectively. Conclusions: We have confirmed that Taxol is as potent inhibitor of fibroblast proliferation as Mitomycin C. This result may possibly support the use of Taxol in trabeculectomy to improve the success rate of trabeculectomy.

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Available abstract

Purpose: Scar tissue formation by proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated inhibitory effects of Mitomycin C (MMC) and Taxol on proliferation of cultured rabbit subconjunctival fibroblasts. Methods: Tenon's fibroblasts of rabbits were cultured and exposed to various concentrations of each drug for 6 days. Cell viability was measured using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumm bromide) assay. Results: Both MMC and Taxol inhibited proliferation of fibroblasts significantly in a dose-dependent manner, with ID50s of 0.27 m/l and 0.08 m/l, respectively. Conclusions: We have confirmed that Taxol is as potent inhibitor of fibroblast proliferation as Mitomycin C. This result may possibly support the use of Taxol in trabeculectomy to improve the success rate of trabeculectomy.

Key concepts: Mitomycin C, Fibroblast, Trabeculectomy, Medicine, Cell growth, MTT assay, Wound healing, Molecular biology

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Inhibitory Effects of Taxol and Mitomycin C on Proliferation of Cultured Rabbit Subconjunctival Fibroblasts — Research Paper | ScholarLens