2018•ScholarSpace - JCCC (Johnson County Community College)Requires access

Isolation and Testing of Antimicrobial Compounds Produced by Bacteria Closely Related to Bacillus subtilis

Jessalyn Rivet-Tissot

Abstract

Bacillus subtilis has been widely used by biotechnology companies for industrial-scale secreted enzyme production. A close relative of Bacillus subtilis isolated from local soil samples was previously shown to inhibit the growth of E. coli and S. epidermidis when grown competitively using a cross streak method. Organic extracts from this bacteria has been tested against several ESKAPE pathogens using both direct application and TLC–bioautography. Further work to isolate and test the antimicrobial compounds produced by this bacteria closely related to Bacillus subtilis will be discussed.

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Key concepts: Bacillus subtilis, Isolation (microbiology), Antimicrobial, Bacteria, Microbiology, Bacillales, Bacillus (shape), Bacillaceae