The Effects of Circumin on Fibroblast Cells and Circumin with Metformin on Fibroblast cells

Previous experiments have been performed testing circumin with gemcitabine on pancreatic cancer but not many others. The results show that the mice which were treated with circumin and gemcitabine suppress proliferation, angiogenesis, NF-κB,and NF-κB–regulated gene products.These results provide further evidence supporting the hypothesis and an experimental basis for using circumin alone. Circumin could stop cancer cell growth and be an effective treatment for pancreatic cancer. Metformin pills were crushed and circumin pills were opened. The water used to dilute the metformin must first be distilled so it is sterile. One metformin pill, 500mg, will be diluted into one liter of water. One circumin pill, 500mg, will be diluted in 10 ml of of water. Each concentration was exposed UV light under a fume hood for approximately 20 minutes before introducing them to the fibroblasts. The fibroblasts were passaged on CO2-dependent media and incubated to ensure there was a high enough count of viable cells to perform the tests on. While the cells were growing, they had to be “fed” or given nutrients through FBS (fetal bovine serum). 10% FBS and 1% antibiotics were added to the media. Then, the entire solution of media was filtered to ensure no extra particles or bacterium got into the plates. Once the cells were plated and allowed to grow for a 48 hour period, the circumin alone, metformin and circumin together, metformin alone, paclitaxel alone were introduced for an exposure time of 48 hours. Next, 125 microliters of paclixtal was added in the 1st row of wells, 125 microliters of metformin in the 2nd row, 125 microliters of circumin in the 3rd row, 62.5 microliters of metformin and circumin together, and a control with 1 mL of media in a different plate. Assays were run and results showed combination metformin and circumin to have the highest viability while circumin alone had the highest percentage of cell death.