2019IOP Conference Series Materials Science and EngineeringOpen access

Isolation and Identification of Bacteria and Actinomycetes Isolated from Wilting Banana Plants (Musa Sp.)

Saryono, P. Finna, P. Usman, P. N. Wahyu, A. Aulia

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Abstract

Abstract In Indonesia, wilting disease in banana due toFusariumhas spread in several areas. In addition, fungi, bacteria, and actinomycetes are also known to cause the disease. In this research, isolation and identification of bacteria and actinomysetes causing the wilting of rot and rhizosphere were done. Identification used several stages of biochemical tests. There were 40 bacterial isolates with 9 generas, which werePseudomonassp.,Xanthomonassp.,Caulobactersp.,Erwiniasp.,Eschericiasp.,Ralstoniasp.,Neisseriasp.,Staphylococcussp., andProteussp. Whilst 18 isolates of actinomycetes were obtained with 7 generas, includingNocardiasp.,Actinobiosporasp.,Nocardiopsissp.,Streptomycessp.,Streptoverticilliumsp.,Streptosporangiumsp., andMicrobisporasp.

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Abstract In Indonesia, wilting disease in banana due toFusariumhas spread in several areas. In addition, fungi, bacteria, and actinomycetes are also known to cause the disease. In this research, isolation and identification of bacteria and actinomysetes causing the wilting of rot and rhizosphere were done. Identification used several stages of biochemical tests. There were 40 bacterial isolates with 9 generas, which werePseudomonassp.,Xanthomonassp.,Caulobactersp.,Erwiniasp.,Eschericiasp.,Ralstoniasp.,Neisseriasp.,Staphylococcussp., andProteussp. Whilst 18 isolates of actinomycetes were obtained with 7 generas, includingNocardiasp.,Actinobiosporasp.,Nocardiopsissp.,Streptomycessp.,Streptoverticilliumsp.,Streptosporangiumsp., andMicrobisporasp.

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Available abstract

Abstract In Indonesia, wilting disease in banana due toFusariumhas spread in several areas. In addition, fungi, bacteria, and actinomycetes are also known to cause the disease. In this research, isolation and identification of bacteria and actinomysetes causing the wilting of rot and rhizosphere were done. Identification used several stages of biochemical tests. There were 40 bacterial isolates with 9 generas, which werePseudomonassp.,Xanthomonassp.,Caulobactersp.,Erwiniasp.,Eschericiasp.,Ralstoniasp.,Neisseriasp.,Staphylococcussp., andProteussp. Whilst 18 isolates of actinomycetes were obtained with 7 generas, includingNocardiasp.,Actinobiosporasp.,Nocardiopsissp.,Streptomycessp.,Streptoverticilliumsp.,Streptosporangiumsp., andMicrobisporasp.

Key concepts: Biology, Wilting, Microbiology, Bacteria, Rhizosphere, Nocardia, Streptomyces, Fusarium

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