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2019•PubMedOpen access

Design and Construction of a Eukaryotic Cloning Vector Encoding the mpt51 Gene of Mycobacterium tuberculosis.

Faria Hasanzadeh Haghighi, Ehsan Aryan, Aida Gholoobi, Hosna Zare, Zahra Meshkat

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Abstract

BACKGROUND: gene. METHODS: TOP10 strain were transformed by the recombinant plasmid. Positive clones were identified by colony PCR, restriction enzyme digestion, and DNA sequencing. RESULTS: fragment of H37Rv in GenBank. CONCLUSION: gene of MTB was correctly cloned into pcDNA3.1(+). The expression of this recombinant vector can be studied in eukaryotic cells. Moreover, it is possible to determine the efficacy of this vector as a DNA vaccine candidate, and to test its protective function compared to BCG in animal models in future.

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BACKGROUND: gene. METHODS: TOP10 strain were transformed by the recombinant plasmid. Positive clones were identified by colony PCR, restriction enzyme digestion, and DNA sequencing. RESULTS: fragment of H37Rv in GenBank. CONCLUSION: gene of MTB was correctly cloned into pcDNA3.1(+). The expression of this recombinant vector can be studied in eukaryotic cells. Moreover, it is possible to determine the efficacy of this vector as a DNA vaccine candidate, and to test its protective function compared to BCG in animal models in future.

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Available abstract

BACKGROUND: gene. METHODS: TOP10 strain were transformed by the recombinant plasmid. Positive clones were identified by colony PCR, restriction enzyme digestion, and DNA sequencing. RESULTS: fragment of H37Rv in GenBank. CONCLUSION: gene of MTB was correctly cloned into pcDNA3.1(+). The expression of this recombinant vector can be studied in eukaryotic cells. Moreover, it is possible to determine the efficacy of this vector as a DNA vaccine candidate, and to test its protective function compared to BCG in animal models in future.

Key concepts: Restriction enzyme, Recombinant DNA, Biology, Gene, Cloning vector, Plasmid, Cloning (programming), Vector (molecular biology)

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Design and Construction of a Eukaryotic Cloning Vector Encoding the mpt51 Gene of Mycobacterium tuberculosis. — Research Paper | ScholarLens