Construction of the Fused Eukaryotic Expression Vector of Mycobacterium tuberculosis mpt64 Gene and Its Expression

In order to construction the fused eukaryotic expression vector of Mycobacterium tuberculosis mpt64 gene and express it in COS-7 cell.The gene encoding mpt64 protein was amplified by polymerase chain reaction(PCR)from genome of Mycobacterium tuberculosis H37Rv strain,and first inserted into cloning vector pGEM-T-easy.After sequencing was confirmed,the gene was subcloned to eukaryotic expression vector pCDNA3.1(-).The recombinant plasmid pCDNA-mpt64 was transfected into COS-7 cells with liposome.The expression was detected by RT-PCR and indirect immunofluorescence.Eukaryotic recombinant plasmid encoding mpt64 were constructed successfully.The mpt64 gene can be expressed in COS-7 cell.