1998•Europe PMC (PubMed Central)Requires access
A preliminary study on transfection of tumor-infiltrating lymphocytes from human glioma with tumor necrosis factor alpha gene
Huaqun Chen, Youzhang Wu, Ni Li, Hong‐Fu Li, Wenhong Wang, Xiaojian Ding, Chengya Wang
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Abstract
Expressible TNF alpha gene was inserted into pLXSN retroviral vector through BamH I site. The resulting construct was transfected into PA317 packing line with lipofectin method, and high titer (1.4×10~(6)CFU/L) of recombinant virus was obtained. We infected tumor-infiltrating cells isolated from human glioma biopsies with retrovirus and obtained G418 resistant cells. Expressed TNF in culture supernatant of viral infected TIL cells was detectable both by ELISA and L929 dependent cells. The TNF-alpha expressible recombinant virus prepared can infect TIL cells from human glioma driven the TNF-alpha gene expression effectively.