STUDY OF ANDROGRAPHOLIDE CONTENT IN ANDROGRAPHIS PANICULATA FROM DIFFERENT FOREST TYPES OF MADHYA PRADESH, INDIA

This study aimed to develop and validate the content of andrographolide content in Andrographis paniculata from different forest types of Madhya Pradesh India.Total six samples of Andrographis paniculata were collected from three forest types of Madhya Pradesh.Sample were analysed through HPLC chromatographic method.HPLC instrument that was used for the estimation of andrographolide were of the following features, HPLC-grade waters, Pump -515 Isocratic pump, Injector -Rheodyne injector with a 20-microlitre loop, Detector -UV Vis detector, Software -Data ace software, Column -Thermo C-18 column (4.6 x 250mm, 5μ particle size), sample size (20µl).Highest percentage of Andrographolide in this plant were from Bamhni forests accounts to 0.658%, followed by Barhi 0.459%, Amarvada 0.124%, Panpatha 0.101%, Amarpur 0.071%, and Kurrai 0.061%.There was considerable variation in the percentage of andrographolide from one site to another site of different forest types of Madhya Pradesh.Considerable variation in the content of andrographolide was studied from one forest to another forest.