2013•Letters in BiotechnologyRequires access
Triptolide Induces Apoptosis of Human Pancreatic Cancer Cells
Qiao Ming Zhi
Open publisher page 0 citations
Abstract
Objective:To determine whether triptolide inhibits proliferation and induces apoptosis of pancreatic cancer cells.Methods:The effects of triptolide on the antiproliferative activity of pancreatic cancer cells BxPC-3 and PANC-1 were determined by MTT assays.Apoptosis of pancreatic cancer cells induced by triptolide were de termined by flow cytometry analysis.Mitochondrial membranere potential and ROS production analysis were detect ed by Rhodamine 123 and DCFH-DA.The effects of triptolide on expression levels of Bcl-2 and Bax were deter mined by Western blotting.Results:Triptolide displayed inhibition activity of cells growth and induction of apopto sis on pancreatic cancer cells.Triptolide induced the loss of mitochondrial membrane potential by regulating the expression levels of Bcl-2 family proteins.Conclusion:The results suggest that triptolide may inhibit proliferation and induce apoptosis of pancreatic cancer cells by inducing the loss of mitochondrial membrane potential.