Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain

Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is required