Improving and experiencing of semi-section with resin embedded

[Objective] To improve the staining technic of semi-sections with resin embedded and to be applied in pathology diagnosis and fine structure study.[Methods]The semi-sections with resin embedded were stained using multicolor and single staining methods.[Results] In multicolor staning sections there were contrast,similar to haematoxylin and eosin staining,then It can be used in many kinds of tissues for staining.Single staining sections were absence of levels and no contract,but it was better for nerve and muscle tissues.[Conclusions ]It was clear,fresh and like haematoxylin and eosin staining in multicolor semi-sections with resin embedded,it had integration characters with high resolving and many kinds optics staining,the best paraffin sections were cannot compare with it in cellular fine structra observed.