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2019•International Journal of Current Microbiology and Applied SciencesOpen access

Biocontrol Potential of Non-pathogenic Fusarium Isolates against the Fusarium Wilt of Chrysanthemum

M. Swathi, L. Krishna Naik

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Abstract

Non-pathogenic Fusarium isolates were isolated and five isolates were selected for their biocontrol activity against Fusarium wilt of chrysanthemum. Different methods of in vitro tests were conducted, under the dual plate method the isolate UASB NPF-III and UASB NPF-I inhibited the pathogen by 64.17 % and 60.44 % respectively at seventh day of incubation. The cell free culture filtrates of non pathogenic Fusarium isolates UASB NPF-III and UASB NPF-I were found effective in controlling the pathogen by 26.36 % and 24.34 % at seventh day of inoculation. Similarly, the methanol and ethyl extract of culture filtrate of UASB NPF-III also have some effects on the pathogen and the inhibition per cent was about 41.81 % and 39.99 % respectively.

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Non-pathogenic Fusarium isolates were isolated and five isolates were selected for their biocontrol activity against Fusarium wilt of chrysanthemum. Different methods of in vitro tests were conducted, under the dual plate method the isolate UASB NPF-III and UASB NPF-I inhibited the pathogen by 64.17 % and 60.44 % respectively at seventh day of incubation. The cell free culture filtrates of non pathogenic Fusarium isolates UASB NPF-III and UASB NPF-I were found effective in controlling the pathogen by 26.36 % and 24.34 % at seventh day of inoculation. Similarly, the methanol and ethyl extract of culture filtrate of UASB NPF-III also have some effects on the pathogen and the inhibition per cent was about 41.81 % and 39.99 % respectively.

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Available abstract

Non-pathogenic Fusarium isolates were isolated and five isolates were selected for their biocontrol activity against Fusarium wilt of chrysanthemum. Different methods of in vitro tests were conducted, under the dual plate method the isolate UASB NPF-III and UASB NPF-I inhibited the pathogen by 64.17 % and 60.44 % respectively at seventh day of incubation. The cell free culture filtrates of non pathogenic Fusarium isolates UASB NPF-III and UASB NPF-I were found effective in controlling the pathogen by 26.36 % and 24.34 % at seventh day of inoculation. Similarly, the methanol and ethyl extract of culture filtrate of UASB NPF-III also have some effects on the pathogen and the inhibition per cent was about 41.81 % and 39.99 % respectively.

Key concepts: Pathogen, Biology, Inoculation, Fusarium, Biological pest control, Fusarium wilt, Incubation, Microbiology

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