Effect of drying temperatures and extraction solvents on total phenolic, flavonoid contents and antioxidant properties of immature Manis Terengganu Melon (Cucumis melo)
Norhazilah Muhamad, W Sahadan, Lee Hoon Ho
Abstract
Norhazilah Muhamad, W Sahadan, Lee Hoon Ho
Abstract
The aims of this study was to determine the effects of drying temperature (40 °C, 50 °C and 60 °C) on the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity on immature Manis Terengganu Melon. The extraction was performed using different solvent (water, methanol and ethanol). The TPC, TFC and antioxidant activity were determined by using Folin-Ciocalteu method, Aluminium Chloride method, (2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Free Reducing Antioxidant Power (FRAP assay), respectively. Drying temperature at 40 °C showed the highest amount of TPC and TFC extract. However, drying temperature at 50 and 60 °C showed the higher DPPH and FRAP values. By comparing the extraction solvents, methanol is the most efficient solvent to produce the highest value of TFC, DPPH and FRAP. Meanwhile, the used of water extract resulted in higher TPC amount compared to ethanol and methanol. The present obtained result may be used in food industries for developing of functional food ingredient
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The aims of this study was to determine the effects of drying temperature (40 °C, 50 °C and 60 °C) on the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity on immature Manis Terengganu Melon. The extraction was performed using different solvent (water, methanol and ethanol). The TPC, TFC and antioxidant activity were determined by using Folin-Ciocalteu method, Aluminium Chloride method, (2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Free Reducing Antioxidant Power (FRAP assay), respectively. Drying temperature at 40 °C showed the highest amount of TPC and TFC extract. However, drying temperature at 50 and 60 °C showed the higher DPPH and FRAP values. By comparing the extraction solvents, methanol is the most efficient solvent to produce the highest value of TFC, DPPH and FRAP. Meanwhile, the used of water extract resulted in higher TPC amount compared to ethanol and methanol. The present obtained result may be used in food industries for developing of functional food ingredient
Key concepts: DPPH, Chemistry, Flavonoid, Extraction (chemistry), Melon, Methanol, Food science, Antioxidant