2018•Applied Ecology and Environmental ResearchOpen access

EXPLORATION OF SOIL MICROBIAL DIVERSITY IN RHIZOSPHERE OF SOME TRITICEAE SPECIES IN SHANXI, CHINA

Zhu Yang, Wen‐Ju Yang, J. HAO, Mingzhou Sun, Z. Q. GAO, Shahbaz Khan, Sumera Anwar, Taisheng Du, N XUE

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Abstract

Microbial diversity in rhizospheric soil plays a vital role for sustainable crop production and maintenance of soil quality.The aim of this study was to evaluate microbial diversity in rhizosphere of Triticeae species by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing technique and to select suitable varieties being introduced to Shanxi province to improve soil quality and crop production.In the experiment, there were nine introduced varieties of Triticeae crops and one local breeding winter wheat variety (S1, Shannong 129, white grain, middle-gluten).The nine Triticeae varieties included six common wheat varieties, one durum wheat variety (S8, Yavaros F9), one rye variety (S9, rye 4R) and one triticale variety (S10, taisi 1).The six common wheat varieties were: Yannong 19 (S2, white grain, middle-gluten, winter), Huaimai 18 (S3, white, middle-gluten, half-winter), Yumai 34 (S4, white, high-gluten, spring), Shannong zixiaomai (S5, purple, middle-gluten, winter), Heixiaomai 031244 (S6, black, high-gluten, winter), Wumai 202W22 (S7, black, high-gluten, spring).Results showed that the rhizospheric bacterial and fungal community varied with Triticeae genera and wheat varieties.Huaimai 18 stimulated the increase of uncultured bacterium and Nabis ferus, while Shannong 129 stimulated the increase of uncultured Chloroflexi bacterium.Bacterial diversity was higher in rhizosphere of Heixiaomai 031244, whereas, fungal diversity was relatively higher in Huaimai 18.Hence, our study implies that, Huaimai 18, Yannong 19, and Heixiaomai 031244 could be planted in Shanxi province to develop high quality wheat variety resources and rye 4R as a feed crop for planting structure adjustment.

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Microbial diversity in rhizospheric soil plays a vital role for sustainable crop production and maintenance of soil quality.The aim of this study was to evaluate microbial diversity in rhizosphere of Triticeae species by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing technique and to select suitable varieties being introduced to Shanxi province to improve soil quality and crop production.In the experiment, there were nine introduced varieties of Triticeae crops and one local breeding winter wheat variety (S1, Shannong 129, white grain, middle-gluten).The nine Triticeae varieties included six common wheat varieties, one durum wheat variety (S8, Yavaros F9), one rye variety (S9, rye 4R) and one triticale variety (S10, taisi 1).The six common wheat varieties were: Yannong 19 (S2, white grain, middle-gluten, winter), Huaimai 18 (S3, white, middle-gluten, half-winter), Yumai 34 (S4, white, high-gluten, spring), Shannong zixiaomai (S5, purple, middle-gluten, winter), Heixiaomai 031244 (S6, black, high-gluten, winter), Wumai 202W22 (S7, black, high-gluten, spring).Results showed that the rhizospheric bacterial and fungal community varied with Triticeae genera and wheat varieties.Huaimai 18 stimulated the increase of uncultured bacterium and Nabis ferus, while Shannong 129 stimulated the increase of uncultured Chloroflexi bacterium.Bacterial diversity was higher in rhizosphere of Heixiaomai 031244, whereas, fungal diversity was relatively higher in Huaimai 18.Hence, our study implies that, Huaimai 18, Yannong 19, and Heixiaomai 031244 could be planted in Shanxi province to develop high quality wheat variety resources and rye 4R as a feed crop for planting structure adjustment.

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Available abstract

Microbial diversity in rhizospheric soil plays a vital role for sustainable crop production and maintenance of soil quality.The aim of this study was to evaluate microbial diversity in rhizosphere of Triticeae species by denaturing gradient gel electrophoresis (DGGE) and DNA sequencing technique and to select suitable varieties being introduced to Shanxi province to improve soil quality and crop production.In the experiment, there were nine introduced varieties of Triticeae crops and one local breeding winter wheat variety (S1, Shannong 129, white grain, middle-gluten).The nine Triticeae varieties included six common wheat varieties, one durum wheat variety (S8, Yavaros F9), one rye variety (S9, rye 4R) and one triticale variety (S10, taisi 1).The six common wheat varieties were: Yannong 19 (S2, white grain, middle-gluten, winter), Huaimai 18 (S3, white, middle-gluten, half-winter), Yumai 34 (S4, white, high-gluten, spring), Shannong zixiaomai (S5, purple, middle-gluten, winter), Heixiaomai 031244 (S6, black, high-gluten, winter), Wumai 202W22 (S7, black, high-gluten, spring).Results showed that the rhizospheric bacterial and fungal community varied with Triticeae genera and wheat varieties.Huaimai 18 stimulated the increase of uncultured bacterium and Nabis ferus, while Shannong 129 stimulated the increase of uncultured Chloroflexi bacterium.Bacterial diversity was higher in rhizosphere of Heixiaomai 031244, whereas, fungal diversity was relatively higher in Huaimai 18.Hence, our study implies that, Huaimai 18, Yannong 19, and Heixiaomai 031244 could be planted in Shanxi province to develop high quality wheat variety resources and rye 4R as a feed crop for planting structure adjustment.

Key concepts: Triticeae, Rhizosphere, China, Diversity (politics), Botany, Biology, Geography, Bacteria

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