Intervention effects of Hellgrammites (Megaloptera: Corydalidae) on ‘Kidney-Yang Deficiency syndrome’ rats

The present study was conducted to explore the effects of hellgrammites protein hydrocortisone administration induced Kidney-Yang Deficiency syndrome (KYDS) rats. The proteins were extracted from hellgrammites. Protein content was determined by Kjeldahl method. 51 rats were divided randomly into 5 groups. (group 1: blank control; group 2: KYDS model; group 3: KYDS rats treated with desmopressin(DDAVP); group 4: KYDS rats treated with hellgrammites protein low dose group 100 mg/kg; group 5: KYDS rats treated with hellgrammites protein high dose group 200 mg/kg). The rats were injected intraperitoneally hydrocortisone once daily for 18 days to simulate ‘Kidney-Yang Deficiency syndrome’, then the ninth day administered orally hellgrammites protein extract once daily for the following 12 days. The ALD, AVP, CAMP, CAMP/CGMP and renal tissue expression in different groups were compared. Compared with blank control diet group, CAMP/CGMP in hyperlipemia model group decreased ( P < 0. 05) and increased ALD(P ＞0.05). After treatment with hellgrammites protein high dose group and DDAVP group, CAMP/CGMP of the Kidney yang deficiency rats increased (P< 0. 05) and increased ALD(P＜0. 05). The hellgrammites protein extract have protective effects on the rat model of kidney yang deficiency and polyuria caused by hydrocortisone.