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2014•International Journal of Marine ScienceRequires access

Genetic Diversity of GIFT Tilapia Populations by AFLP Analysis

Tao Yan, Yusong Guo, Zhongduo Wang, Li Liu, Liu ChuWu

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Abstract

The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia (GIFT) in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou (Guangdong, China) was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers (E-AGG/M-CTT, E-AGG/M-CTG, E-AAC/M-CAG, E-ACA/M-CTG, E-ACA/M-CAG) based on 27 individuals, and among them 163 (87.17%) were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei’s gene diversity was 0.288,6, average Shannon’s information index was 0.433,9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.

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What this paper is about

The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia (GIFT) in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou (Guangdong, China) was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers (E-AGG/M-CTT, E-AGG/M-CTG, E-AAC/M-CAG, E-ACA/M-CTG, E-ACA/M-CAG) based on 27 individuals, and among them 163 (87.17%) were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei’s gene diversity was 0.288,6, average Shannon’s information index was 0.433,9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.

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Available abstract

The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia (GIFT) in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou (Guangdong, China) was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers (E-AGG/M-CTT, E-AGG/M-CTG, E-AAC/M-CAG, E-ACA/M-CTG, E-ACA/M-CAG) based on 27 individuals, and among them 163 (87.17%) were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei’s gene diversity was 0.288,6, average Shannon’s information index was 0.433,9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.

Key concepts: Amplified fragment length polymorphism, Genetic diversity, Evolutionary biology, Diversity (politics), Biology, Zoology, Population, Sociology

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