GC/MS Fingerprint Analysis of Osmanthus fragrans Lour.in Different Varieties
Hui Cao, Zuguang Li, Shen De-long
Abstract
Hui Cao, Zuguang Li, Shen De-long
Abstract
The headspace constituents emitted from different varieties of fresh Osmanthus fragrans Lour.flowers were investigated by means of headspace solid-phase microextraction(HS-SPME)coupled to gas chromatography/mass spectrometry(GC/MS).In this study 20 volatile compounds were identified in Osmanthus fragrans var.thunbergii,13 volatile compounds were identified in Osmanthus fragrans,9 volatile compounds were identified in Osmanthus fragrans f.aurantiacus.(Z)-ocimene,4-hexenyl acetate,cis-linalool oxide,trans-linalool oxide,β-linalool,α-ionone,β-ionone,γ-decalactone were the same volatiles released from fresh flowers of three different Osmanthus fragrans Lour.The fingerprints were analyzed by similarity evaluation and principle component analysis to conduct the chemical pattern recognition.The results indicated that headspace SPME-GC/MS afforded a simple sampling method for identification of the main volatile fragrance from different varieties of fresh Osmanthus fragrans Lour.flowers.The fragrans of Osmanthus fragrans Lour.flowers not only had some resemblance,but also had some difference.The different varieties of fresh Osmanthus fragrans Lour.flowers were distinguished and classified by fingerprints similarity evaluation and principle component analysis.
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The headspace constituents emitted from different varieties of fresh Osmanthus fragrans Lour.flowers were investigated by means of headspace solid-phase microextraction(HS-SPME)coupled to gas chromatography/mass spectrometry(GC/MS).In this study 20 volatile compounds were identified in Osmanthus fragrans var.thunbergii,13 volatile compounds were identified in Osmanthus fragrans,9 volatile compounds were identified in Osmanthus fragrans f.aurantiacus.(Z)-ocimene,4-hexenyl acetate,cis-linalool oxide,trans-linalool oxide,β-linalool,α-ionone,β-ionone,γ-decalactone were the same volatiles released from fresh flowers of three different Osmanthus fragrans Lour.The fingerprints were analyzed by similarity evaluation and principle component analysis to conduct the chemical pattern recognition.The results indicated that headspace SPME-GC/MS afforded a simple sampling method for identification of the main volatile fragrance from different varieties of fresh Osmanthus fragrans Lour.flowers.The fragrans of Osmanthus fragrans Lour.flowers not only had some resemblance,but also had some difference.The different varieties of fresh Osmanthus fragrans Lour.flowers were distinguished and classified by fingerprints similarity evaluation and principle component analysis.
Key concepts: Osmanthus fragrans, Linalool, Chemistry, Gas chromatography–mass spectrometry, Botany, Chromatography, Horticulture, Mass spectrometry