2009•Anhui Nongye Daxue xuebaoRequires access
Cloning and sequence analysis of interferon-α gene of Zangao canine.
Hong‐Ying Chen, Haiju Dong, Gui BaoAn, Yanyan Jia, Song YaPeng, Shujuan Wang
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Abstract
One pair of primers was designed and synthesized according to the canine interferon alpha gene(IFN-α) nucleic acid sequence(AB102731) published in the GenBank.Canine IFN-α gene was amplified by PCR from genome DNA extracted directionally from liver of Zangao canine,then cloned and sequenced.The result showed the full length sequence of Zangao canine IFN-α gene was consisted of 564 bp,which includes one open-reading frame,encoding 187 amino acid residues,three potential N-glycosalation sites and ten cysteines in deduced amino acid sequence.The results of comparative sequence analysis and phylogenetic tree analysis showed there was difference of genus in IFN-α gene,the nearer relationship,the higher homology.This study set a basis the way for future study of biological function and application of canine IFN-α.