Cloning and bioinformatics analysis of IFN-αgene of wild cat

One pair of primer was designed and synthesized according to the feline interferon alpha gene(IFN-α) nucleic acid sequence.Wild cat IFN-α was amplified by PCR,then cloned and sequenced.The nucleotide sequence of the amplified IFN-α gene and the amino acid sequence deduced from the gene were then analyzed by the DNAstar software and on-line tools,and the similarity and evolution of IFN-α gene were analyzed among wild cat,human and other animals.The results showed the full length sequence of wild cat IFN-α gene was 631 bp,which includes one open-reading frame,encoding 194 amino acid residues,one potential N-glycosalation site and seven O-glcnac sites in deduced amino acid sequence.The results of comparative sequence analysis and phyloge-netic tree analysis indicated there was difference of genus in IFN-α gene,the nearer their relationship,the higher the homology.This study paved the way for future study of biological function and application of feline IFN-α.