EFFECT OF CHLOROGENIC ACID ON GLUCOSE CONSUMPTION IN HepG2 CELLS PRETREATED WITH HIGH INSULIN AND HIGH OLEIC ACID

Objective To explore the modulation of chlorogenic acid (CGA) on glucose metabolism in HepG2 cells pretreated with high insulin and high oleic acid (OA). Methods Cultured HepG2 cells induced by high insulin and oleic acid for insulin resistance and steatosis respectively, were co-cultured with different concentrations of CGA (10,20,40,80 mg/L) for 24h. The morphological changes were observed and glucose consumptions of cells were measured by glucose oxidase method. Results Compared to control group, CGA could significantly increase glucose consumption of normal HepG2 cells and the dosedependent effect was noted between 10-40 mg/L(P0.05). The glucose consumption of HepG2 cells in insulin resistance (IR) group was decreased by 39.5% (P0.05), but increased by 60.5%-93.5% as compared to IR group (P0.05),after the cells were treated by CGA, most potent in 20mg/L CGA group. The glucose consumption of HepG2 cells in OA group was decreased by 26.2% (P0.05), but increased by 27.3%-63.5% as compared to OA group (P0.05),after the cells were treated by CGA, also most potent in 20 mg/L CGA group. Conclusion CGA can improve glucose metabolic disturbance induced by high insulin and high oleic acid.