2018•Cold Spring Harbor ProtocolsRequires access

Analysis of Cell Viability by the alamarBlue Assay

Priti Kumar, Arvindhan Nagarajan, Pradeep D. Uchil

Abstract

This protocol describes viability measurements for cell cultures in a 96-well tissue culture plate using alamarBlue (resazurin). The assay can be modified to accommodate larger plates; however, for a preliminary analysis of transfection reagents and parameters of the transfection protocol on cell viability, a 96-well plate format is the most cost effective.

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Available abstract

Key concepts: Resazurin, Viability assay, Transfection, Protocol (science), Cell, Chemistry, Cell culture, Biology