2018•South African Journal of BotanyOpen access

Phenolic acids contribution to antioxidant activities and comparative assessment of phenolic content in mango pulp and peel

Snežana Agatonović-Kuštrin, Ella Kustrin, David William Morton

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Abstract

A high performance thin layer chromatography (HPTLC) method to analyse antioxidant activity as free-radical scavenging activity and metal-chelation in selected mango varieties was developed and validated. The contributions of common phenolic acids to antioxidant activity in pulp and peel samples was assessed. Polyphenolic content in pulp and peel was correlated with chlorogenic and gallic acid concentration ( R > 0.90). Free radical scavenging activity in the peel was related to gallic and chlorogenic acids ( R = 0.83), but wasn't dependent on caffeic acid concentration. Free radical scavenging activity in the pulp could not be predicted from the gallic or chlorogenic acid ( R = 0.25 and 0.45) indicating that pulp contains other, more powerful free radical scavengers.

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A high performance thin layer chromatography (HPTLC) method to analyse antioxidant activity as free-radical scavenging activity and metal-chelation in selected mango varieties was developed and validated. The contributions of common phenolic acids to antioxidant activity in pulp and peel samples was assessed. Polyphenolic content in pulp and peel was correlated with chlorogenic and gallic acid concentration ( R > 0.90). Free radical scavenging activity in the peel was related to gallic and chlorogenic acids ( R = 0.83), but wasn't dependent on caffeic acid concentration. Free radical scavenging activity in the pulp could not be predicted from the gallic or chlorogenic acid ( R = 0.25 and 0.45) indicating that pulp contains other, more powerful free radical scavengers.

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Available abstract

A high performance thin layer chromatography (HPTLC) method to analyse antioxidant activity as free-radical scavenging activity and metal-chelation in selected mango varieties was developed and validated. The contributions of common phenolic acids to antioxidant activity in pulp and peel samples was assessed. Polyphenolic content in pulp and peel was correlated with chlorogenic and gallic acid concentration ( R > 0.90). Free radical scavenging activity in the peel was related to gallic and chlorogenic acids ( R = 0.83), but wasn't dependent on caffeic acid concentration. Free radical scavenging activity in the pulp could not be predicted from the gallic or chlorogenic acid ( R = 0.25 and 0.45) indicating that pulp contains other, more powerful free radical scavengers.

Key concepts: Gallic acid, Chlorogenic acid, Chemistry, Caffeic acid, Pulp (tooth), Polyphenol, Antioxidant, Food science

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