Early diagnosis of infection occurs in burned patients and verification in vitro

Abstract Objective To explore the trend of CD64+ neutrophils being present in the peripheral blood of patients with burns and verification in vitro. Method We isolated and purified CD64+ neutrophils from healthy people, stimulated cells with inactivated Staphylococcus aureus in vitro to make them proliferate and analyzed them with Modfit 2 analysis software. Patients with burns were divided into two groups, depending on whether there was a complicated infection or not, and 35 healthy participants were enrolled as blank group. WBC and CD64+ neutrophil counts were detected and then analyzed the specificity and sensitivity according to the ROC curve. Results The proliferation index of the CD64+ cells in the experimental wells was significantly higher than that in the control wells (P < .05). The CD64+ cell counts in the peripheral blood of patients in the infection group were significantly higher than patients without infection, but also higher than the healthy people (P < .05). The sensitivity and specificity of CD64+ cellular diagnosis for burn infection were 94.2% and 76.8%, respectively, according to the ROC curve, which were significantly better than those of the WBCs. Conclusion CD64+ cells in the peripheral blood of patients with burns complicated by infection increased significantly, and thus, it can be regarded as an early diagnostic indicator of burns with a concurrent infection.