Cloning of MSTN Gene of Landes Goose and Research of Relationship Between MSTN Gene Expression and Different Energe Diets, or IGF-I and GH in Serum

Objective The myostatin gene of Landes goose was amplified by reverse transcription-polymerase chain reaction (RT-PCR),and the relationship between MSTN gene expression and different energy diets,or IGF-I,GH levels in serum was researched in order to know the function of MSTN gene and provide information for molecular nutriology and breeding of water fowl. MethodGenome RNA was extracted from muscle of Landes goose,MSTN gene mRNA was amplified by RT-PCR,the PCR product was cloned into pGEM-T vector and transformed into Escherichia coli. The MSTN gene was isolated and sequenced from the positive clones screened. Based on the MSTN gene clone,β-actin as inner control,an optimal semi-quantitative RT-PCR method was constructed to study the difference of MSTN gene expression at 21st and 70th day of Landes goose fed with different energy diets,and IGF-I and GH in serum were determined. Result The unique DNA fragment about 1 128 bp in length was obtained.Sequence analysis revealed that it has a length of 1128 nucleotides which encodes a peptide of 375 amino acids. Compared with homologous sequence of chicken,duck and goose,it displayed a fairly high degree conservation. The homology of the nucleotide sequence were 94%,94%,and 99%,respectively; the homology of the amino acid sequence were 98%,97%,and 98%,respectively; at 21st day and 70th day,the results showed that the expression of MSTN in female Landes geese was a little higher than male at 21st day; the expression of MSTN in male was ACB at 70th day,female was CBA at 70th day; In the period of 21-70 days,the expression of MSTN was changed with IGF-I in serum; it was not associated with GH in serum,GH was significantly correlated with energy. Conclusion Energy influences MSTN gene expression of Landes goose after 21 days; and MSTN gene expression is associated with IGF-I in serum,but it is not associated with GH in serum.