Cloning of MSTN Gene and Research of Relationship between MSTN Gene Expression and Different Energy Diets or IGF-I,GH in Serum of Xupu Geese

Genome RNA was extracted from leg muscle of Xupu geese,MSTN gene mRNA was amplified by using RT-PCR,the PCR product was cloned into pGEM-T vector.The MSTN gene was isolated and sequenced from the positive clones screened based on the MSTN gene clone,using β-actin as inner control,constructing an optimal semi-quantitative RT-PCR method,the difference of MSTN gene expression at 21 and 70 days by feeding different energy diets,at one time we detected IGF-I and GH in serum.The unique DNA fragment about 1 128 bp in length was obtained.Sequence analysis revealed that it has a length of 1 128 bp which encodes a 375 amino peptide.Compared with homologous sequence of chicken,duck and Landes geese,it displayed a fairly high conservation.The homologue of the nucleotide sequence are 94%,94%,99%,respectively;the homologue of the amino acid sequence deduced are 98%,97%,98%,respectively.The results showed the expressions of MSTN in Xupu geese were showed no significantly differences at 21 days;but the expression of MSTN was CBA by 70 days;During 21-70 days period,the expression of MSTN was changed with IGF-I in serum;it was not associated with GH in serum.The different energy influenced MSTN gene expsession of Xupu geese after 21 days.The present study laid a foundation for further investigating MSTN gene in waterfowl.